Construction of AC/DC magnetic syringe device for stimulated drug release, injection and ejection of nanocarriers and testing cytotoxicity in vitro

Milorad Zjalić; Mislav Mustapić; Zvonko Glumac; Ivan Prološčić; Senka Blažetić; Ana Vuković; Mostafa Masud; Motasim Billah; Aslam Khan; Suzana Šegota; Md Shahriar Al Hossain; Marija Heffer

doi:10.1016/j.mex.2021.101312

Construction of AC/DC magnetic syringe device for stimulated drug release, injection and ejection of nanocarriers and testing cytotoxicity in vitro

MethodsX. 2021 Mar 21:8:101312. doi: 10.1016/j.mex.2021.101312. eCollection 2021.

Authors

Affiliations

¹ Department of Medical Biology and Genetics, Faculty of Medicine, J.J. Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia.
² Department of Physics, J.J. Strossmayer University of Osijek, Trg Ljudevita Gaja 6, Osijek 31000, Croatia.
³ Department of Biology, J.J. Strossmayer University of Osijek, Ulica cara Hadrijana 8a, Osijek 31000, Croatia.
⁴ Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Brisbane QLD 4067, Australia.
⁵ School of Mechanical and Mining Engineering, University of Queensland, St. Lucia, Brisbane QLD 4067, Australia.
⁶ King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.
⁷ Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb 10000, Croatia.

Abstract

Iron nanoparticles are used as a targeted drug delivery system. The nanocarrier itself can be genotoxic, trigger oxidative stress or cell death. Therefore, we developed an AC/DC magnetic syringe for injecting, stimulating drug release and safe removing of the nanocarrier. Alongside we optimized the method for nanoparticles' drug release kinetics and testing cytotoxicity in vitro.•This paper presents detailed instructions for construction of AC/DC magnetic syringe device for stimulated drug release, injection and ejection of magnetic nanoparticles; nanoparticles preparation; adsorbing methylene blue on nanoparticles; determination of drug release kinetics from nanocarriers on the example of methylene blue•Gomori´s Prussian blue reaction for differentiated SH-SY5Y human neuroblastoma cell line; MTT viability assay optimized for differentiated SH-SY5Y human neuroblastoma cell line and antioxidant enzymes activities assay and lipid peroxidation methods are optimized for cell analyses cell cultivation for nanoparticles cytotoxicity testing in vitro.•Those protocols are the first step toward further testing the effect of nanoparticles in vivo, on brain tissue.

Keywords: Cell viability; Drug delivery systems; Lipid peroxidation; Metal nanoparticles; Oxidative stress.