Method for high-throughput antifungal activity screening of bacterial strain libraries

Jeffrey D Palumbo; Teresa L O'Keeffe

doi:10.1016/j.mimet.2021.106311

Method for high-throughput antifungal activity screening of bacterial strain libraries

J Microbiol Methods. 2021 Oct:189:106311. doi: 10.1016/j.mimet.2021.106311. Epub 2021 Aug 21.

Authors

Jeffrey D Palumbo¹, Teresa L O'Keeffe²

Affiliations

¹ Foodborne Toxin Detection and Prevention Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Albany, CA, USA. Electronic address: jeffrey.palumbo@usda.gov.
² Foodborne Toxin Detection and Prevention Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Albany, CA, USA.

PMID: 34428496
DOI: 10.1016/j.mimet.2021.106311

Abstract

Our laboratory developed a high-throughput screen for antifungal phenotypes in which bacterial libraries in 96-well format are transferred to agar pour plates inoculated with spores of the target fungus. This method is an improvement over bacterial/fungal coculture plate methods that measure antifungal activity as inhibition of radial fungal growth.

Keywords: Antagonism; Antibiosis; Biocontrol; Coculture.

Published by Elsevier B.V.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Antibiosis
Antifungal Agents / pharmacology*
Bacteria / classification
Bacteria / drug effects*
Fungi / classification
Fungi / drug effects*
High-Throughput Screening Assays / methods*
Microbial Sensitivity Tests

Substances

Antifungal Agents