Exosomal DNMT1 mRNA transcript is elevated in acute lymphoblastic leukemia which might reprograms leukemia progression

Cancer Genet. 2022 Jan:260-261:57-64. doi: 10.1016/j.cancergen.2021.07.004. Epub 2021 Aug 1.

Abstract

DNMT1 (DNA-methyltransferase 1) is an enzyme which contributes to the process of normal embryonic development, and aberrant expression of DNMT1 leads to tumor/leukemia progression by inducing significant changes in DNA methylation and epigenetics. We found that DNMT1 mRNA transcript is elevated in Exo-PALL compared to Exo-HD. We also confirmed and showed heightened levels of DNMT1 mRNA transcript in Exo-CM of leukemia cell lines. Co-culture of Exo-PALL with target cells (leukemia B cells) showed transfer of exosomal DNMT1 mRNA transcript into the target cells, which may reprogram the biological nature of normal healthy cells and leukemia cells.

Keywords: Acute lymphocytic leukemia; Epigenetics, DNMT1; Exosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Child
  • Coculture Techniques
  • DNA (Cytosine-5-)-Methyltransferase 1 / genetics*
  • Disease Progression
  • Epigenesis, Genetic
  • Exosomes / genetics*
  • Gene Expression Regulation, Neoplastic
  • HEK293 Cells
  • Humans
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Up-Regulation*

Substances

  • DNA (Cytosine-5-)-Methyltransferase 1
  • DNMT1 protein, human