The up-regulation of miR-21 by gastrodin to promote the angiogenesis ability of human umbilical vein endothelial cells by activating the signaling pathway of PI3K/Akt

Bioengineered. 2021 Dec;12(1):5402-5410. doi: 10.1080/21655979.2021.1964895.

Abstract

Studies have shown that gastrodin has a protective effect on blood vessels. The purpose of this study was to investigate the influence of gastrodin on the angiogenesis ability of human umbilical vein endothelial cells (HUVECs) and its mechanism. We found that treatment of HUVECs with 10 µM and 25 µM gastrodin, and Vascular endothelial growth factor (VEGF) significantly upregulated the miR-21 expression in the cells. Meanwhile, gastrodin significantly increased the cell proliferation, migration and tube formation ability of HUVECs and increased the expression of MMP-2 and MMP-9 mRNA. In addition, gastrodin promoted the phosphorylation level of PI3K/Akt protein. However, down-regulating the miR-21 expression reduced the promoting effect of gastrodin on the HUVECs angiogenesis. In conclusion, gastrodin activates the PI3K/Akt pathway by up-regulating the miR-21 expression and promotes the HUVECs angiogenesis.

Keywords: Gastrodin; angiogenesis; huvecs; miR-21; pi3k/akt signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzyl Alcohols / pharmacology*
  • Cells, Cultured
  • Glucosides / pharmacology*
  • Human Umbilical Vein Endothelial Cells / drug effects*
  • Human Umbilical Vein Endothelial Cells / metabolism
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Neovascularization, Physiologic / drug effects*
  • Phosphatidylinositol 3-Kinases / genetics
  • Phosphatidylinositol 3-Kinases / metabolism
  • Proto-Oncogene Proteins c-akt / genetics
  • Proto-Oncogene Proteins c-akt / metabolism
  • Signal Transduction / drug effects*
  • Up-Regulation / drug effects

Substances

  • Benzyl Alcohols
  • Glucosides
  • MIRN21 microRNA, human
  • MicroRNAs
  • gastrodin
  • Proto-Oncogene Proteins c-akt

Grants and funding

National Administration of Traditional Chinese Medicine: Evidence-Based Capacity Building Project(2019XZZX-NB007); 333 high level talents training project in Jiangsu (Grant No. BRA 2016507); Jiangsu Province Administration of Chinese Medicine (ZX2016A2).