Mineralization of lead by Phanerochaete chrysosporium microcapsules loaded with hydroxyapatite

Abstract

In this study, microcapsules assembled with Phanerochaete chrysosporium (P. chrysosporium, PC) and hydroxyapatite (HAP) were successfully prepared and applied for Pb(II) immobilization in aqueous solution. The effect of different conditions on Pb(II) removal was investigated, such as pH, temperature, dosages of microcapsules and HAP, and initial concentrations of Pb(II). The removal efficiency of Pb(II) was in order of HAP+PC > HAP > PC > CK (control check) at the Pb(II) initial concentration of 100 mg L^-1, which were 87.7%, 82.82%, 63.67% and 2.06%, respectively. Under HAP+PC treatment, P. chrysosporium secreted plentiful organic acids like formic, oxalic and citric acids, when the addition dose of HAP increased from 5 g L^-1 to 15 g L^-1, the production of formic acid increased remarkably from 32.37 g L^-1 to 66.02 g L^-1. After reaction, P. chrysosporium kept a good biological activity evidenced by the live/dead stain test. The characterization results indicated that the insoluble apatite could transform to soluble phosphate due to the secreted organic acids, then reacted with Pb(II) to form pyromorphite [Pb₁₀(PO₄)₆Cl₂] and lead phosphate hydroxide [Pb₁₀(PO₄)₆(OH)₂]. The overall results clearly demonstrated that combining P. chrysosporium with HAP could be used as a promising technology to accelerate lead immobilization.

Keywords: Biomineralization; Hydroxyapatite; Lead; Microcapsules; Organic acids; Phanerochaete chrysosporium.