Enzymatic production of lactulose by fed-batch and repeated fed-batch reactor

Abstract

The effects of the most significant operational variables on reactor performance of fed-batch and repeated fed-batch were evaluated in the lactulose production by enzymatic transgalactosylation. Feed flowrate in the fed stage (F) and fructose to lactose molar ratio (Fr/L) were the variables that mostly affected the values ​​of lactulose yield (Y_Lu), lactulose productivity (π_Lu) and selectivity of transgalactosylation (S_Lu/_TOS). Maximum Y_Lu of 0.21 g lactulose per g lactose was obtained at 50% w/w inlet carbohydrates concentration (IC) of, 50 °C, Fr/L 8, F 1 mL⋅min^-1, 200 IU∙g_Lactose^-1 reactor enzyme load and pH 4.5. At these conditions the selectivity was 7.4, productivity was 0.71 g_Lu∙g^-1∙h^-1and lactose conversion was 0.66. The operation by repeated fed batch increases the efficiency of use of the biocatalysts (E_B) and the accumulated productivity compared to batch and fed batch operation with the same biocatalyst. E_B obtained was 4.13 g_Lu∙mg_{biocatalyst protein}^-1, 10.6 times higher than in fed-batch.

Keywords: Fed-batch; Immobilized enzyme; Lactulose; Repeated fed-batch; β-galactosidase.