Vergence and accommodation can be mismatched under virtual reality viewing conditions, and this mismatch has been thought to be one of the main causes of visual discomfort. The goal of this study was to investigate how optical conditions of the eyes affect accommodative responses to different convergence. Specifically, we hypothesized that extending the depth of focus (DoF) could weaken the control of the screen on accommodation, so that accommodation could be induced by convergence. To test this hypothesis, we extended the DoF using Zernike spherical aberrations (fourth and sixth orders) induced by a binocular adaptive optics (AO) vision simulator. Nine normal subjects between the ages of 21 and 34 (26 ± 5) years were recruited. Three optical conditions were generated: AO condition (aberration-free), monovision condition, and extended depth of focus (EDoF) condition. Binocular accommodative responses, along with binocular visual acuity and stereoacuity, were measured under all three optical conditions with varied binocular vergence levels. At 3 diopters of binocular convergence, the EDoF condition was the most efficient in inducing excessive accommodative response compared with the monovision condition and the AO condition. Visual acuity was impaired with EDoF as compared with the other two conditions. The average stereoscopic thresholds (at 0 vergence) under the EDoF condition were degraded compared with the AO condition but were superior to those of the monovision condition. Therefore, despite some compromise to visual performance, extending the DoF could allow for a more natural vergence-accommodation relationship, providing the potential for alleviating the vergence-accommodation conflict and associated visual fatigue symptoms in virtual reality.