Intelligent pH-responsive dental sealants to prevent long-term microleakage

Hao Li; Yuyao Huang; Xuedong Zhou; Chengguang Zhu; Qi Han; Haohao Wang; Hockin H K Xu; Biao Ren; Lei Cheng

doi:10.1016/j.dental.2021.08.002

Intelligent pH-responsive dental sealants to prevent long-term microleakage

Dent Mater. 2021 Oct;37(10):1529-1541. doi: 10.1016/j.dental.2021.08.002. Epub 2021 Aug 16.

Authors

Hao Li¹, Yuyao Huang¹, Xuedong Zhou¹, Chengguang Zhu¹, Qi Han², Haohao Wang¹, Hockin H K Xu³, Biao Ren⁴, Lei Cheng⁵

Affiliations

¹ State Key Laboratory of Oral Diseases, West China School of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, 610041, China; Department of Operative Dentistry and Endodontics, West China School of Stomatology, Sichuan University, Chengdu, 610041, China.
² State Key Laboratory of Oral Diseases, West China School of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, 610041, China; Department of Oral Pathology, West China School of Stomatology, Sichuan University, Chengdu, 610041, China.
³ Department of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA. Electronic address: Hxu@umaryland.edu.
⁴ State Key Laboratory of Oral Diseases, West China School of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, 610041, China. Electronic address: renbiao@scu.edu.cn.
⁵ State Key Laboratory of Oral Diseases, West China School of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, 610041, China; Department of Operative Dentistry and Endodontics, West China School of Stomatology, Sichuan University, Chengdu, 610041, China. Electronic address: chenglei@scu.edu.cn.

PMID: 34412907
DOI: 10.1016/j.dental.2021.08.002

Abstract

Objectives: Microleakage is a determinant factor of failures in sealant application. In this study, DMAEM (dodecylmethylaminoethyl methacrylate), a pH-responsive antibacterial agent, was incorporated into resin-based sealant for the first time. The objectives of this study were to: (1) investigate the long-term performance of DMAEM-modified sealants against oral microbial-aging; and (2) investigate the long-term preventive effect of DMAEM-modified sealants on microleakage.

Methods: Depth-of-cure and cytotoxicity of DMAEM-modified sealants were measured. Then, an aging model using biofilm derived from the saliva of high caries experience children was conducted. After aging, microhardness and surface roughness were measured. Biofilm activity, lactic acid production and exopolysaccharide (EPS) production were measured. 16S rRNA gene sequencing were also performed. The effects of DMAEM on microleakage were tested using an in vitro microleakage assessment.

Results: The addition of DMAEM with a mass fraction of 2.5-10% did not affect depth-of-cure values and cytotoxicity of sealants. Adding 2.5-10% DMAEM did not affect the surface roughness and microhardness after aging. Compared to control, adding 2.5-10% DMAEM reduced biofilm metabolic activity by more than 80%. The lactic acid production and EPS production were reduced by 50% in DMAEM groups. DMAEM-modified sealants maintained the microbial diversity of biofilm after aging, they also inhibited the growth of lactobacillus. The 5% and 10% DMAEM groups exhibited a significant reduction in microleakage compared to control.

Significance: The long-term antibacterial activities against oral microbial-aging and the long-term microecosystem-regulating capabilities enabled DMAEM-modified sealant to prevent microleakage in sealant application and thus prevent dental caries.

Keywords: Dental caries; Dodecylmethylaminoethyl methacrylate; Microbial-aging; Microleakage; Resin-based sealant; pH response.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biofilms
Child
Dental Caries* / prevention & control
Dental Leakage* / prevention & control
Humans
Hydrogen-Ion Concentration
Pit and Fissure Sealants
RNA, Ribosomal, 16S

Substances

Pit and Fissure Sealants
RNA, Ribosomal, 16S