Ibuprofen inhibits oral NaCl response through transmembrane channel-like 4

Yoichi Kasahara; Masataka Narukawa; Tomoya Nakagita; Keiko Abe; Takumi Misaka; Tomiko Asakura

doi:10.1016/j.bbrc.2021.07.082

Ibuprofen inhibits oral NaCl response through transmembrane channel-like 4

Biochem Biophys Res Commun. 2021 Oct 8:573:76-79. doi: 10.1016/j.bbrc.2021.07.082. Epub 2021 Aug 17.

Authors

Yoichi Kasahara¹, Masataka Narukawa², Tomoya Nakagita³, Keiko Abe⁴, Takumi Misaka¹, Tomiko Asakura⁵

Affiliations

¹ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
² Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan; Department of Food and Nutrition, Kyoto Women's University, 35 Kitahiyoshicho Imakumano, Higashiyama-ku, Kyoto, 605-8501, Japan.
³ Department of Agricultural Chemistry, School of Agriculture, Meiji University, 1-1-1, Higashimita, Kawasaki, Kanagawa, 214-8571, Japan.
⁴ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 210-0821, Japan.
⁵ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. Electronic address: asakura@mail.ecc.u-tokyo.ac.jp.

PMID: 34411898
DOI: 10.1016/j.bbrc.2021.07.082

Abstract

Nonsteroidal anti-inflammatory drugs, such as ibuprofen, are known to modify salty taste perception in humans. However, the underlying molecular mechanisms remain unknown. We investigated the inhibitory effect of ibuprofen on the NaCl stimulation of epithelium sodium channel (ENaC) and transmembrane channel-like 4 (TMC4), which are involved in salty taste detection. Although ibuprofen only minimally inhibited the response of the ENaC to NaCl, it significantly inhibited the TMC4 response to NaCl with an IC₅₀ at 1.45 mM. These results suggest that ibuprofen interferes with detection of salty taste via inhibition of TMC4.

Keywords: ENaC; Ibuprofen; Salty taste; TMC4.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Humans
Ibuprofen / pharmacology*
Membrane Proteins / antagonists & inhibitors*
Membrane Proteins / metabolism
Sodium Chloride / administration & dosage*
Taste Perception / drug effects

Substances

Anti-Inflammatory Agents, Non-Steroidal
Membrane Proteins
TMC4 protein, human
Sodium Chloride
Ibuprofen