Odor composition of field versus laboratory desert locust populations

Abstract

Olfaction plays an important role in the behavioural ecology of the desert locust Schistocerca gregaria (Orthoptera:Acrididae). Different locust life stages and sexes use olfactory cues for different behaviours such as grouping, mating, oviposition, feeding, maturation and gregarization, which can be exploited for management of the desert locust. However, the full spectrum of the chemistry of volatiles released by the desert locust remains unknown. Here, we compared the volatile emissions of different life stages of a natural wild population reared in the laboratory for one generation with those of a population that has been reared in the laboratory for seven generations. Coupled gas chromatography-mass spectrometric analysis was used to identify captured volatiles. Analysis of similarities (ANOSIM) and non-metric multidimensional scaling of the volatile organic compounds (VOCs) showed significant chemo-diversity between different life stages, and sexes for the adults of the two populations. Additionally, chemical analysis showed that nymphs and adults of the field population released ~4- to 40-fold more volatiles, and they were compositionally richer than their laboratory counterparts. These results demonstrate the composition and variation in odors of field and laboratory populations of the desert locust, and that the discriminating odors warrant further investigation to determine their roles in the bioecology and management of this locust species.

Keywords: Acrididae; Desert locust; Olfaction; Orthoptera; Semiochemicals; Volatiles.