Long-term seasonal and temporal changes of hydrogen peroxide from cyanobacterial blooms in fresh waters

Abstract

In water, hydrogen peroxide (H₂O₂) is produced through abiotic and biotic reactions with organic matter, including algal cells. The production of H₂O₂ is influenced by harmful algal cell communities and toxicity. However, only a few studies have been conducted on H₂O₂ concentrations in natural water. Particularly, the seasonal and temporal patterns of H₂O₂ concentration suggest that H₂O₂ generation from aquatic microorganisms could be identified to compare of photochemical production from dissolved organic matter. Study area is a source of raw water and is a large artificial lake located near a metropolitan city. Due to various environmental conditions, harmful algal blooms frequently occur in summer. The purpose of this study was to trace the H₂O₂ concentration and water quality parameters of study area where algal bloom occurs and what factors directly affect the H₂O₂ concentration. Experiments were performed on the influencing factors via water samples from study area and lab-scale culture tank. The lake produces an average of 553 nM H₂O₂, which increases by more than three times (1460 nM) in summer compared the winter. The lake (18.6-23.8 nMh^-1) produced more H₂O₂ than streams (7.4-9.0 nMh^-1) during daylight hours. All water sites presented the lowest production rates in dark conditions (1.1-1.5 nMh^-1). Daytime environment increased the generation rate more than the nighttime. The trend of H₂O₂ produced by algal cells was similar to that of the growth of algal cells. The exposure to external substances (heavy metals and antibiotics) increased the incidence by approximately five times; antibiotics were more influential than heavy metals.

Keywords: Algal bloom; Antibiotic; Cyanobacteria; Heavy metal; Hydrogen peroxide.