An optimized intracerebroventricular injection of CD4+ T cells into mice

Zoe V Taylor; Bishnu Khand; Angel Porgador; Alon Monsonego; Ekaterina Eremenko

doi:10.1016/j.xpro.2021.100725

An optimized intracerebroventricular injection of CD4⁺ T cells into mice

STAR Protoc. 2021 Aug 6;2(3):100725. doi: 10.1016/j.xpro.2021.100725. eCollection 2021 Sep 17.

Authors

Zoe V Taylor^{1

2}, Bishnu Khand^{1

2}, Angel Porgador^{1

2}, Alon Monsonego^{1

2}, Ekaterina Eremenko^{1

3

2}

Affiliations

¹ The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel.
² The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
³ The Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel.

Abstract

The blood-brain barrier acts as a major barrier for the entrance of most therapeutics into the brain, impeding treatment for neurological disorders. Intracerebroventricular (ICV) injection of T cells is a useful tool for cell therapy of neurological disorders including neurodegenerative and neuropsychiatric diseases and brain tumors. Here, we present an optimized ICV injection of T cells with improved injection efficiency at pathological sites within the brain parenchyma. We describe details of the surgical procedure and verification of injection via immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Fisher et al. (2014); Strominger et al., (2018); Mittal et al. (2019); Eremenko et al. (2019).

Keywords: immunology; microscopy; model organisms; neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood-Brain Barrier / immunology
Blood-Brain Barrier / metabolism*
Brain / metabolism
CD4-Positive T-Lymphocytes / immunology
Immunohistochemistry / methods
Infusions, Intraventricular
Injections / methods*
Injections, Intraventricular / methods*
Lymphatic System / immunology
Lymphocyte Count / methods
Mice
Parenchymal Tissue
T-Lymphocytes / immunology