Exploration of the Characteristics of Intestinal Microbiota and Metabolomics in Different Rat Models of Mongolian Medicine

Riao Dao; Dongxing Wu; Huan Wang; Habur Jin; Li Li; Xiquan Fu; Chula Sa; Eerdunchaolu

doi:10.1155/2021/5532069

Exploration of the Characteristics of Intestinal Microbiota and Metabolomics in Different Rat Models of Mongolian Medicine

Evid Based Complement Alternat Med. 2021 Aug 3:2021:5532069. doi: 10.1155/2021/5532069. eCollection 2021.

Authors

Riao Dao^{1

2}, Dongxing Wu¹, Huan Wang¹, Habur Jin³, Li Li⁴, Xiquan Fu¹, Chula Sa¹, Eerdunchaolu¹

Affiliations

¹ Inner Mongolia University for Nationalities, Mongolian Medical College, Tongliao City, Inner Mongolia Autonomous Region 028000, China.
² Department of Psychosomatic Medicine, International Mongolian Medical Hospital of Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia Autonomous Region 010010, China.
³ Affiliated Hospital of Inner Mongolia University for the Nationalities, Rehabilitation Hospital, Tongliao City, Inner Mongolia Autonomous Region 028000, China.
⁴ Second Department of Encephalopathy, Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao City, Inner Mongolia Autonomous Region 028000, China.

Abstract

Background: Mongolian medicine is a systematic theoretical system, which is based on the balance among Heyi, Xila, and Badagan. However, the underlying mechanisms remain unclear. This study aimed to explore the characteristics of intestinal microbiota and metabolites in different rat models of Mongolian medicine.

Methods: After establishing rat models of Heyi, Xila, and Badagan, we integrated 16S rRNA gene sequencing and metabolomics.

Results: Heyi, Xila, and Badagan rats had significantly altered intestinal microbial composition compared with rats in the MCK group. They showed 11, 18, and 8 significantly differential bacterial biomarkers and 22, 11, and 15 differential metabolites, respectively. The glucosinolate biosynthesis pathway was enriched only in Heyi rats; the biosynthesis of phenylpropanoids pathway and phenylpropanoid biosynthesis pathway were enriched only in Xila rats; the isoflavonoid biosynthesis pathway, the glycine, serine, and threonine metabolism pathway, and the arginine and proline metabolism pathway were enriched only in Badagan rats.

Conclusions: The intestinal microbiota, metabolites, and metabolic pathways significantly differed among Heyi, Xila, and Badagan rats compared with control group rats.