Specific detection of memory T-cells in COVID-19 patients using standardized whole-blood Interferon gammarelease assay

Eur J Immunol. 2021 Dec;51(12):3239-3242. doi: 10.1002/eji.202149296. Epub 2021 Sep 6.

Abstract

Antigen-specific T-cells are essential for protective immunity against SARS-CoV-2. We set up a semi-automated whole-blood Interferon-gamma release assay (WB IGRA) to monitor the T-cell response after stimulation with SARS-CoV-2 peptide pools. We report that the WB IGRA is complementary to serological assays to assess SARS-CoV-2 immunity.

Keywords: SARS-CoV-2; T-cells response; immune memory; interferon gamma release assay.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Automation, Laboratory
  • COVID-19 / immunology*
  • Cells, Cultured
  • Cohort Studies
  • Female
  • Humans
  • Interferon-gamma / metabolism*
  • Interferon-gamma Release Tests / standards
  • Lymphocyte Activation
  • Male
  • Memory T Cells / immunology*
  • Middle Aged
  • SARS-CoV-2 / physiology*
  • T-Cell Antigen Receptor Specificity
  • Whole Body Imaging
  • Young Adult

Substances

  • Interferon-gamma