Genome-wide identification of alcohol dehydrogenase (ADH) gene family under waterlogging stress in wheat (Triticum aestivum)

Changwei Shen; Jingping Yuan; Xingqi Ou; Xiujuan Ren; Xinhua Li

doi:10.7717/peerj.11861

Genome-wide identification of alcohol dehydrogenase (ADH) gene family under waterlogging stress in wheat (Triticum aestivum)

PeerJ. 2021 Jul 23:9:e11861. doi: 10.7717/peerj.11861. eCollection 2021.

Authors

Changwei Shen¹, Jingping Yuan², Xingqi Ou³, Xiujuan Ren¹, Xinhua Li³

Affiliations

¹ School of Resources and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang, Henan, China.
² School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.
³ School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, China.

Abstract

Background: Alcohol dehydrogenase (ADH) plays an important role in plant survival under anaerobic conditions. Although some research about ADH in many plants have been carried out, the bioinformatics analysis of the ADH gene family from Triticum aestivum and their response to abiotic stress is unclear.

Methods: A total of 22 ADH genes were identified from the wheat genome, and these genes could be divided into two subfamilies (subfamily I and subfamily II). All TaADH genes belonged to the Medium-chain ADH subfamily. Sequence alignment analysis showed that all TaADH proteins contained a conservative GroES-like domain and Zinc-binding domain. A total of 64 duplicated gene pairs were found, and the Ka/Ks value of these gene pairs was less than 1, which indicated that these genes were relatively conservative and did not change greatly in the process of duplication.

Results: The organizational analysis showed that nine TaADH genes were highly expressed in all organs, and the rest of TaADH genes had tissue specificity. Cis-acting element analysis showed that almost all of the TaADH genes contained an anaerobic response element. The expression levels of ADH gene in waterlogging tolerant and waterlogging sensitive wheat seeds were analyzed by quantitative real-time PCR (qRT-PCR). This showed that some key ADH genes were significantly responsive to waterlogging stress at the seed germination stage, and the response of waterlogging tolerant and waterlogging sensitive wheat seeds to waterlogging stress was regulated by different ADH genes. The results may be helpful to further study the function of TaADH genes and to determine the candidate gene for wheat stress resistance breeding.

Keywords: Alcohol dehydrogenase; Expression patterns; Triticum aestivum; Waterlogging stress.

Grants and funding

This research was financially supported by the Major Science and Technology Projects of Henan Province (191110110700) to Xingqi Ou, the Science and Technology Program of Henan Province (202102110159) and the Scientific Research Foundation for High—level Talent (2017034) to Changwei Shen, the Scientific Research Foundation for High—level Talent (103010620001/015) to Jingping Yuan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.