ALKBH10B, an mRNA m6A Demethylase, Modulates ABA Response During Seed Germination in Arabidopsis

Jun Tang; Junbo Yang; Hongchao Duan; Guifang Jia

doi:10.3389/fpls.2021.712713

ALKBH10B, an mRNA m⁶A Demethylase, Modulates ABA Response During Seed Germination in Arabidopsis

Front Plant Sci. 2021 Jul 27:12:712713. doi: 10.3389/fpls.2021.712713. eCollection 2021.

Authors

Jun Tang¹, Junbo Yang¹, Hongchao Duan¹, Guifang Jia¹

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

Abstract

As the most abundant and reversible chemical modification in eukaryotic mRNA, the epitranscriptomic mark N ⁶-methyladenine (m⁶A) regulates plant development and stress response. We have previously characterized that ALKBH10B is an Arabidopsis mRNA m⁶A demethylase and regulates floral transition. However, it is unclear whether ALKBH10B plays a role in abiotic stress response. Here, we found that the expression of ALKBH10B is increased in response to abscisic acid (ABA), osmotic, and salt stress. The alkbh10b mutants showed hypersensitive to ABA, osmotic, and salt stress during seed germination. Transcriptome analysis revealed that the expression of several ABA response genes is upregulated in alkbh10b-1 than that of wild type, indicating ALKBH10B negatively affects the ABA signaling. Furthermore, m⁶A sequencing showed that ABA signaling genes, including PYR1, PYL7, PYL9, ABI1, and SnRK2.2 are m⁶A hypermethylated in alkbh10b-1 after ABA treatment. Taken together, our work demonstrated that ALKBH10B negatively modulates ABA response during seed germination in Arabidopsis.

Keywords: ABA; ALKBH10B; Arabidopsis; N6-methyladenine; osmotic stress; seed germination.