Abstract
Allogenic hematopoietic stem cell transplant (HSCT) recipients are susceptible to any kind of infectious agents including Clostridium difficile. We studied 86 allogenic-HSCT patients who faced diarrhoea while receiving antibiotics. DNA from stool samples were explored for the presence of C. difficile toxin genes (tcdA; tcdB) by multiplex real-time PCR. Results showed nine toxigenic C. difficile amongst which seven were positive for both toxins and two were positive for tcdB. Six of toxigenic C. difficile organisms harbouring both toxin genes were also isolated by toxigenic culture. Clostridium difficile infection was controlled successfully with oral Metronidazole and Vancomycin in the confirmed infected patients.
Keywords:
Clostridium difficile; hematopoietic stem cell transplant; real-time PCR.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / therapeutic use
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Clostridioides difficile / genetics
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Clostridioides difficile / isolation & purification
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Clostridioides difficile / metabolism
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Clostridioides difficile / pathogenicity*
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Clostridium Infections / diagnosis
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Clostridium Infections / drug therapy
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Clostridium Infections / microbiology*
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DNA, Bacterial / genetics
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Diarrhea / diagnosis
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Diarrhea / drug therapy
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Diarrhea / microbiology*
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Drug Therapy, Combination
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Enterotoxins / genetics
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Enterotoxins / metabolism*
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Hematopoietic Stem Cell Transplantation*
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Humans
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Metronidazole / therapeutic use
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Polymerase Chain Reaction
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Treatment Outcome
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Vancomycin / therapeutic use
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Enterotoxins
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Metronidazole
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Vancomycin