Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources

Viruses. 2021 Jul 16;13(7):1383. doi: 10.3390/v13071383.

Abstract

The yellow fever virus vaccine, 17D, was derived through the serial passage of the wild-type (WT) strain Asibi virus in mouse and chicken tissue. Since its derivation, the mechanism of attenuation of 17D virus has been investigated using three 17D substrains and WT Asibi virus. Although all three substrains of 17D have been sequenced, only one isolate of Asibi has been examined genetically and all interpretation of attenuation is based on this one isolate. Here, we sequenced the genome of Asibi virus from three different laboratories and show that the WT strain is genetically homogenous at the amino acids that distinguish Asibi from 17D vaccine virus.

Keywords: 17D vaccine; Asibi; consensus compare; sequencing; wild-type virus; yellow fever.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral / immunology
  • Genetic Variation
  • Genome, Viral*
  • Vaccines, Attenuated
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / immunology
  • Whole Genome Sequencing
  • Yellow Fever Vaccine / immunology*
  • Yellow fever virus / classification
  • Yellow fever virus / genetics*
  • Yellow fever virus / immunology

Substances

  • Antigens, Viral
  • Vaccines, Attenuated
  • Viral Envelope Proteins
  • Yellow Fever Vaccine