Identification of GH17 gene family in Vitis vinifera and expression analysis of GH17 under various adversities

Tariq Pervaiz; Tianhua Liu; Xiang Fang; Yanhua Ren; Xiyang Li; Zhongjie Liu; Muhammad Fiaz; Jinggui Fang; Lingfei Shangguan

doi:10.1007/s12298-021-01014-1

Identification of GH17 gene family in Vitis vinifera and expression analysis of GH17 under various adversities

Physiol Mol Biol Plants. 2021 Jul;27(7):1423-1436. doi: 10.1007/s12298-021-01014-1. Epub 2021 Jun 19.

Authors

Tariq Pervaiz^#^{1

2}, Tianhua Liu^#^{1

3}, Xiang Fang¹, Yanhua Ren^{1

3}, Xiyang Li^{1

3}, Zhongjie Liu^{1

3}, Muhammad Fiaz^{1

3}, Jinggui Fang^{1

3}, Lingfei Shangguan^{1

3}

Affiliations

¹ Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095 People's Republic of China.
² Beijing Advanced Innovation Center for Tree Breeding By Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083 People's Republic of China.
³ Fruit Crop Genetic Improvement and Seedling Propagation Engineering, Center of Jiangsu Province, Nanjing, 210095 People's Republic of China.

^# Contributed equally.

Abstract

Glycoside hydrolase (GH, EC 3.2.1) is a group of enzymes that hydrolyzes glycosidic bonds and play a role in the hydrolysis and synthesis of sugars in living organisms. Vitis vinifera is an important fruit crop and it harbors GH17 gene family however, their function in grapes has not been systematically investigated. In this study, a total of 870 GH17 genes were identified from 14 plant species and their structural domain, sequence alignment, phylogenetic tree, collinear analysis, with the expression profiles of VviGH17 gene family was performed. The promoter analysis of VviGH17 gene showed the presence of cis-acting elements, which are responsive to plant growth and development. In addition, elements for plant hormones were found that are triggered in response to abiotic/biological stress. Transcriptomic data led to the identification of several VviGH17 genes, which are associated with bud dormancy and in response to abiotic stress. Transcript analysis was carried out for some of the selected VviGH17 genes RT-qPCR. VviGH17-16 and VviGH17-30 genes were differentially expressed during bud dormancy, fruit development and different abiotic stresses. Moreover, VviGH17-37 and VviGH17-44 were differentially expressed at fruit development, in response to abiotic stress. In addition, subcellular localization predicts that the VviGH17-16, VviGH17-30, and VviGH17-37 genes were located in the cell membrane, while VviGH17-44 gene was located in the vacuole. In conclusion, our study led to the identification of several GH17s and their probable role in development and stress.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-021-01014-1.

Keywords: Abiotic stress; Bud dormancy; GH17 gene family; Vitis vinifera.