On-line SERS detection of bilirubin based on the optofluidic in-fiber integrated GO/Ag NPs for rapid diagnosis of jaundice

Danheng Gao; Xinghua Yang; Pingping Teng; Meng Luo; Haoxin Zhang; Zhihai Liu; Jun Yang; Zhanao Li; Xingyue Wen; Libo Yuan; Kang Li; Mark Bowkett; Nigel Copner

doi:10.1016/j.talanta.2021.122692

On-line SERS detection of bilirubin based on the optofluidic in-fiber integrated GO/Ag NPs for rapid diagnosis of jaundice

Talanta. 2021 Nov 1:234:122692. doi: 10.1016/j.talanta.2021.122692. Epub 2021 Jul 6.

Authors

Danheng Gao¹, Xinghua Yang², Pingping Teng³, Meng Luo¹, Haoxin Zhang⁴, Zhihai Liu¹, Jun Yang¹, Zhanao Li¹, Xingyue Wen¹, Libo Yuan⁵, Kang Li⁶, Mark Bowkett⁶, Nigel Copner⁶

Affiliations

¹ Key Laboratory of In-Fiber Integrated Optics, Ministry of Education, College of Science, Harbin Engineering University, Harbin, 150001, China.
² Key Laboratory of In-Fiber Integrated Optics, Ministry of Education, College of Science, Harbin Engineering University, Harbin, 150001, China. Electronic address: yangxh@hrbeu.edu.cn.
³ Key Laboratory of In-Fiber Integrated Optics, Ministry of Education, College of Science, Harbin Engineering University, Harbin, 150001, China. Electronic address: tengpingping@hrbeu.edu.cn.
⁴ Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin, 150001, China.
⁵ Key Laboratory of In-Fiber Integrated Optics, Ministry of Education, College of Science, Harbin Engineering University, Harbin, 150001, China; Photonics Research Center, Guilin University of Electronics Technology, Guilin, 541004, China.
⁶ Wireless & Optoelectronics Research & Innovation Centre, Faculty of Computing, Engineering & Science, University of South Wales, Wales, CF37 1DL, UK.

PMID: 34364489
DOI: 10.1016/j.talanta.2021.122692

Abstract

In this paper, we propose a self-assembled graphene oxide (GO)/Ag NPs SERS Raman sensor based on a novel type of optofluidic MHF as a point-of-care testing (POCT) device. This device is used to diagnose jaundice and its related diseases through on-line detection of free bilirubin content in human serum. This optofluidic Raman sensor is composed of a microstructured hollow fiber (MHF) with a microstructured channel and a suspended core, which allows the sample solution to flow in the channel while interacting with the strong evanescent field on the suspended core. Here, the suspended core was modified by a GO/Ag NPs SERS substrate. When the sample flows through the channel, and interacts with the strong evanescent field generated by the suspended core, the on-line SERS signal is generated and can be coupled back to the suspended core to be detected. In addition, both the electrostatic interaction and interference between GO/Ag NPs with the target enriched bilirubin. The results show that the detection concentration range of bilirubin aqueous, bilirubin in albumin and bilirubin in human blood are all in the range of 2 μM-100 μM, and all have a good linear response. The limit of detection reaches the order of 10^-6 M. This rapid, sensitive and label-free SERS Raman sensor of free bilirubin in blood can detect excessive levels of bilirubin in the actual blood environment of the human body, providing a broad prospect for clinically accurate diagnosis of jaundice and related diseases.

Keywords: Bilirubin detection; In-fiber Raman senor; Optofluidic microstructured optical fiber.

MeSH terms

Bilirubin
Graphite
Humans
Jaundice*
Metal Nanoparticles*
Silver
Spectrum Analysis, Raman

Substances

graphene oxide
Silver
Graphite
Bilirubin