Chloroplast genome analysis of Chrysotila dentata

Gene. 2021 Dec 15:804:145871. doi: 10.1016/j.gene.2021.145871. Epub 2021 Aug 4.

Abstract

Chrysotila dentata is an ecologically important marine alga contributing to the coccolith formation. In this study, a complete chloroplast (cp DNA) genome of Chrysotila dentata was sequenced by using Illumina Hiseq and was analyzed with the help of a bioinformatics tool CPGAVAS2. The circular chloroplast genome of Chrysotila dentata has a size of 109,017 bp with two inverted repeats (IRs) regions (4513 bp each) which is a common feature in most land plants and algal species. The Chrysotila dentata cp genome consists of 61 identified protein-coding genes, 30 tRNA genes and 6 rRNAs with 21 microsatellites. The phylogenetic relationship with other select algal species revealed a close phylogeny of Chrysotila dentata with Phaeocystis antarctica. This is the first report of the cp genome analysis of genus Chrysotila and the results from this study will be helpful for understanding the genetic structure and function of chloroplast in other species of Chrysotila.

Keywords: Chloroplast; Chrysotila dentata; Genome sequencing; Haplophyta.

MeSH terms

  • Chloroplasts / genetics*
  • Computational Biology / methods
  • Evolution, Molecular
  • Genes, Plant
  • Genome
  • Haptophyta / genetics*
  • High-Throughput Nucleotide Sequencing / methods
  • Inverted Repeat Sequences / genetics
  • Microsatellite Repeats / genetics
  • Phylogeny
  • RNA, Ribosomal / genetics
  • Sequence Analysis, DNA / methods
  • Whole Genome Sequencing / methods

Substances

  • RNA, Ribosomal