Objective: To study the expression of B lymphocyte-induced mature protein-1 (BLIMP-1) in regulatory T cells (Tregs) of children with aplastic anemia (AA), and analyze its correlation with the number of Tregs and the levels of inhibitory cytokines interleukin (IL)-10 and transforming growth factor (TGF)-β in plasma.
Methods: The peripheral blood samples of 10 newly diagnosed AA children and 10 healthy children were collected for experiment. qPCR was used to detect FOXP3 and PRDM1 mRNA expression levels. Flow cytometry was used to detect the proportion of Tregs, the expression of BLIMP-1 in Tregs, and the levels of cytokines such as IL-2, IL-17A, IL-6, interferon (IFN)-γ, IL-10 and TGF-β in plasma. Pearson correlation model was used to evaluate the relationship between the expression of BLIMP-1 in Treg and the number of Tregs, as well as the levels of IL-10 and TGF-β in plasma.
Results: Compared with control group, the proportion of Tregs in peripheral blood of AA children was decreased significantly (P<0.001); The plasma levels of proinflammatory cytokines IL-2, IL-6 and IFN-γ in AA children were increased significantly (P=0.033, P=0.031, P=0.006), and IL-17A also was increased but the difference was not statistically significant (P=0.052), while anti-inflammatory cytokines IL-10 and TGF-β were significantly reduced (P=0.048, P=0.002). The relative expressions level of FOXP3 and PRDM1 mRNA in AA children were significantly lower than those in control group (P=0.037, P=0.016). The expression of BLIMP-1 protein in Tregs of AA children was significantly lower than that in control group (P<0.001). The expression level of BLIMP-1 protein in Tregs was positively correlated with the percentage of Tregs in lymphocytes (r=0.671, P=0.001), and was also positively correlated with the levels of IL-10 and TGF-β in plasma (r=0.500, P=0.029; r=0.486, P=0.030).
Conclusion: The expression of BLIMP-1 in Tregs of AA children is impaired, and the low expression of BLIMP-1 is related to the decrease of the number in Tregs and IL-10 and TGF-β expressions.
题目: BLIMP-1在再生障碍性贫血患儿Treg细胞中的表达及意义.
目的: 研究B淋巴细胞诱导成熟蛋白-1（BLIMP-1）在再生障碍性贫血（AA）患儿Treg细胞中的表达并分析其与Treg细胞数量、血浆中抑制性细胞因子白介素（IL）-10和转化生长因子（TGF）-β水平的相关性.
方法: 收集10例初诊AA患儿和10例健康儿童的外周血标本。采用qPCR法检测FOXP3、PRDM1的mRNA表达水平。采用流式细胞术检测Treg细胞比例、Treg细胞中BLIMP-1表达以及血浆中IL-2、IL-17A、IL-6、γ干扰素、IL-10和TGF-β等细胞因子水平。采用Pearson相关模型分析Treg细胞中BLIMP-1表达与Treg细胞数量、血浆中IL-10和TGF-β水平的相关性.
结果: 与对照组相比，AA患儿外周血Treg细胞比例显著下降（P＜0.001）；AA患儿血浆中促炎细胞因子IL-2、IL-6、IFN-γ水平显著升高（P=0.033，P=0.031，P=0.006），IL-17A水平亦升高但不显著（P=0.052），而抑炎细胞因子IL-10和TGF-β水平明显降低（P=0.048，P=0.002）；AA患儿FOXP3和PRDM1 mRNA的相对表达量显著低于对照组（P=0.037，P=0.016）；AA患儿Treg细胞的BLIMP-1蛋白表达显著低于对照组（P＜0.001）。Treg细胞中BLIMP-1蛋白的表达水平与Treg细胞占淋巴细胞百分比呈正相关（r=0.671，P=0.001)，与血浆中IL-10和TGF-β水平亦呈正相关（r=0.500，P=0.029；r=0.486，P=0.030).
结论: AA患儿Treg细胞中BLIMP-1表达受损，且BLIMP-1表达低下与Treg细胞数量减少和IL-10、TGF-β水平减少具有相关性.