A new dicistro-like virus from soldier fly, Inopus flavus (Diptera: Stratiomyidae), a pest of sugarcane

Arch Virol. 2021 Oct;166(10):2841-2846. doi: 10.1007/s00705-021-05171-2. Epub 2021 Aug 6.

Abstract

Native Australian soldier flies, Inopus spp. (Diptera: Stratiomyidae), are agricultural pests of economic importance to the sugarcane industry. A screen of the salivary gland transcriptome of Inopus flavus (James) revealed the presence of viral RNA belonging to a potentially novel member of the family Dicistroviridae. The complete genome sequence consists of 9793 nucleotides with two open reading frames. The genome includes two potential internal ribosomal entry sites (IRESs): one within the 5' UTR and the other in the intergenic region (IGR). Virus particles purified from infected larvae and visualised by electron microscopy were found to be icosahedral, non-enveloped, and 30 nm in diameter.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Australia
  • Dicistroviridae / classification*
  • Dicistroviridae / genetics
  • Diptera / virology*
  • Genetic Variation
  • Genome, Viral / genetics
  • Internal Ribosome Entry Sites / genetics
  • Larva / virology
  • Open Reading Frames / genetics
  • Phylogeny
  • RNA, Viral / genetics
  • Saccharum / parasitology*
  • Salivary Glands / virology
  • Virion / ultrastructure

Substances

  • Internal Ribosome Entry Sites
  • RNA, Viral