Generation and live imaging of tumors with specific genotypes in the living fly pupa

STAR Protoc. 2021 Jul 19;2(3):100672. doi: 10.1016/j.xpro.2021.100672. eCollection 2021 Sep 17.

Abstract

This protocol describes the step-by-step generation of tumors with specific genotypes on the dorsal thorax epithelium of the fly. This in vivo system allows the imaging of tumor cell morphology and behavior in high spatial and temporal resolution. Phenotypes such as cell invasion, cell division, and tumor size can be quantified and compared to specific controls or to the neighboring wild-type tissue. Thus, this model allows the study of conserved genes that enhance or suppress epithelial tumor progression. For complete details on the use and execution of this protocol, please refer to Canales Coutiño et al. (2020).

Keywords: Cancer; Cell Biology; Genetics; Microscopy; Model Organisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Crosses, Genetic
  • Drosophila Proteins / genetics
  • Drosophila melanogaster / genetics*
  • Gene Expression Regulation, Neoplastic
  • Genotype
  • Green Fluorescent Proteins / genetics
  • Microscopy, Confocal / methods*
  • Neoplasms, Experimental / genetics*
  • Neoplasms, Experimental / pathology*
  • Pupa* / genetics
  • Tumor Suppressor Proteins / genetics

Substances

  • Drosophila Proteins
  • Tumor Suppressor Proteins
  • l(2)gl protein, Drosophila
  • Green Fluorescent Proteins