A practical guide to photoswitching optoacoustics tomography

Abstract

Photochromic proteins and photoswitching optoacoustics (OA) are a promising combination, that allows OA imaging of even small numbers of cells in whole live animals and thus can facilitate a more wide-spread use of OA in life-science and preclinical research. The concept relies on exploiting the modulation achieved by the photoswitching to discriminate the agents' signal from the non-modulating background. Here we share our analysis approaches that can be readily used on data generated with commercial OA tomography imaging instrumentation allowing-depending on the used photoswitching agent and sample-routine visualizations of as little as several hundreds of transgene labeled cells per imaging volume in the live animal.

Keywords: Bacteriophytochrome; Image analysis; Imaging; Optoacoustic; Photoacoustic; Photochromic protein; Photoswitching; Transgene reporter; Unmixing.