Polymorphism analysis and expression patterns of the IGF1 gene in the Shitou goose

Jun Tang; Mao Guo; Jing Fu; Hongjia Ouyang; Yunbo Tian; Xu Shen; Yunmao Huang

doi:10.5194/aab-64-315-2021

Polymorphism analysis and expression patterns of the IGF1 gene in the Shitou goose

Arch Anim Breed. 2021 Jul 22;64(2):315-323. doi: 10.5194/aab-64-315-2021. eCollection 2021.

Authors

Jun Tang^{1

2}, Mao Guo¹, Jing Fu¹, Hongjia Ouyang^{1

2}, Yunbo Tian^{1

2}, Xu Shen^{1

2}, Yunmao Huang^{1

2}

Affiliations

¹ College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. China.
² Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong, 510225, P.R. China.

Abstract

Insulin-like growth factor 1 (IGF1) is one of the endocrine hormones that plays an important role in regulating growth and development of animals. In this study, polymorphism in the 5 $^{'}$ UTR and 3 $^{'}$ UTR coding region and of the IGF1 gene was detected by DNA sequencing technology, and the abundance of IGF1 mRNA in various tissues at three growth stages of the Shitou goose was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, the differential expression of IGF1 in various tissues between the Shitou goose and Wuzong goose was revealed. Two single nucleotide polymorphisms (SNPs) were found in the exon3 region of IGF1 in the Shitou goose. IGF1 mRNA was extensively expressed in various tissues of Shitou geese with high abundant expression in the liver, breast muscle and leg muscle at three growth stages. IGF1 mRNA expression showed a trend of first increase and then decrease in the pituitary, liver, subcutaneous fat and abdominal fat tissues, but it decreased in the breast muscle and leg muscle of a Shitou goose with growing age. Expression of IGF1 in the liver, leg muscle and pituitary tissues of the Shitou goose was significantly higher than that of the Wuzong goose. This provides a foundation for further study of regulatory mechanisms of IGF1 in the growth and development of geese.