Effect of Yiqi Huoxue Granules on Platelet Activation Induced by Thrombin

Zhen Lei; Shuibo Gao; Xinzhou Wang; Haixia Gao; Yongjun Han; Zhentao Wang; Hong Wu

doi:10.1155/2021/6622848

Effect of Yiqi Huoxue Granules on Platelet Activation Induced by Thrombin

Evid Based Complement Alternat Med. 2021 Jul 16:2021:6622848. doi: 10.1155/2021/6622848. eCollection 2021.

Authors

Zhen Lei^{1

2}, Shuibo Gao^{1

2}, Xinzhou Wang^{1

2}, Haixia Gao^{1

2}, Yongjun Han^{1

2}, Zhentao Wang³, Hong Wu^{1

2

3}

Affiliations

¹ Central Laboratory, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450002, China.
² Laboratory of Cell Imaging, Henan University of Chinese Medicine, Zhengzhou 450002, China.
³ Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou 450002, China.

Abstract

Objective: To study the effects of Yiqi Huoxue (YQHX) granules on platelet activation and aggregation induced by thrombin.

Methods: The effect of YQHX on platelet aggregation rate was detected by platelet aggregation instrument; the effect of YQHX on thrombosis time was observed by the mouse mesentery thrombosis model. DAMI cells were induced to transform into platelet-like granules using PMA, and the effects of SCH (PAR-1 inhibitor) on thrombin-induced changes in platelet intracellular calcium concentration, PAR-1 protein expression, and phosphorylation of MAPK were examined.

Results: Compared with the control group, the platelet aggregation rate, PAR-1 protein expression, phosphorylation of ERK1/2, and p38 protein in the YQHX group decreased (P < 0.05), and there was no significant difference between the YQHX + SCH group and YQHX group (P > 0.05).

Conclusion: YQHX suppresses the platelet activation induced by thrombin by inhibiting PAR-1 expression.