Enhanced bone tissue regeneration via bioactive electrospun fibrous composite coated titanium orthopedic implant

Qichun Song; Selvakani Prabakaran; Jiafeng Duan; Murugaraj Jeyaraj; Suresh Mickymaray; Anand Paramasivam; Mariappan Rajan

doi:10.1016/j.ijpharm.2021.120961

Enhanced bone tissue regeneration via bioactive electrospun fibrous composite coated titanium orthopedic implant

Int J Pharm. 2021 Sep 25:607:120961. doi: 10.1016/j.ijpharm.2021.120961. Epub 2021 Jul 29.

Authors

Qichun Song¹, Selvakani Prabakaran², Jiafeng Duan³, Murugaraj Jeyaraj⁴, Suresh Mickymaray⁵, Anand Paramasivam⁶, Mariappan Rajan⁷

Affiliations

¹ Department of Orthopaedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
² Biomaterials in Medicinal Chemistry Laboratory, Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, India. Electronic address: prabakarans193@gmail.com.
³ Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology Xi'an Jiaotong University, Xi'an 710004, China.
⁴ University of Madras, Guindy Campus, Chennai 25, Tamil Nadu, India.
⁵ Department of Biology, College of Science, Al-Zulfi, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia.
⁶ Department of Basic Medical Sciences, College of Dentistry, Al-Zulfi, Majmaah University, Majmaah 11952, Riyadh region, Saudi Arabia.
⁷ Biomaterials in Medicinal Chemistry Laboratory, Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, India.

PMID: 34333026
DOI: 10.1016/j.ijpharm.2021.120961

Abstract

One of the very reliable, attractive, and cheapest techniques for synthesizing nanofibers for biomedical applications is electrospinning. Here, we have created a novel nanofibrous composite coated Ti plate to mimic an Extra Cellular Matrix (ECM) of native bone in order to enhance the bone tissue regeneration. An electrospun fibrous composite was obtained by the combination of minerals (Zn, Mg, Si) substituted hydroxyapatite (MHAP)/Polyethylene Glycol (PEG)/Cissus quadrangularis (CQ) extract. Fibrous composite's functionality, phase characteristics, and morphology were evaluated by FT-IR, XRD, and SEM techniques, respectively. The average fiber diameter of MHAP/PVA had decreased from ~274 to ~255 nm after incorporating PEG polymer. That further increased from ~255 to ~275 nm after adding CQ extract. Besides the bioactivity in SBF solution, the degradable nature was confirmed by immersing the fibrous composite in Tris-HCL solution. The degradable studies evaluate that the composite was degraded depending on time, and it degrades about 9.42% after 7 days of immersion. Osteoblasts like MG-63 cells differentiation, proliferation, and calcium deposition were also determined. These results show that this new fibrous composite exhibits advanced osteoblasts properties. Thus, we concluded that this new fibrous scaffold coated Ti implant could act as a better implant to mimic ECM of bone structure and to improve osteogenesis during bone regeneration.

Keywords: Calcium nitrate tetrahydrate (Pub Chem CID: 16211656); Cissus quadrangularis; Diammonium hydrogen phosphate (Pub Chem CID: 24540); Electrospinning; Extra Cellular Matrix; Magnesium nitrate hexahydrate (Pub Chem SID: 329752449); Nanofibers; Nitric acid (Pub chem CID: 944); Polyvinyl alcohol (Pub Chem CID: 11199); Sodium Sulfate (Pub Chem CID: 24436); Sodium bicarbonate (Pub Chem CID: 516892); Sulfuric acid (Pub Chem CID: 1118); Tetraethyl orthosilicate (Pub Chem CID: 6517); Zinc nitrate hexahydrate (Pub Chem CID: 15865313).

MeSH terms

Bone Regeneration
Bone and Bones
Cell Proliferation
Durapatite
Nanofibers*
Osteoblasts
Spectroscopy, Fourier Transform Infrared
Tissue Scaffolds
Titanium*

Substances

Durapatite
Titanium