Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue

Philip E Lapinski; Philip D King

doi:10.1007/978-1-0716-1480-8_8

Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue

Methods Mol Biol. 2021:2319:69-75. doi: 10.1007/978-1-0716-1480-8_8.

Authors

Philip E Lapinski¹, Philip D King²

Affiliations

¹ Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
² Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA. kingp@umich.edu.

PMID: 34331244
DOI: 10.1007/978-1-0716-1480-8_8

Abstract

There is increasing interest in the study of the mammalian lymphatic system, including the lymphatic endothelial cells (LECs) that make up lymphatic vessels. The ability to isolate primary LECs from tissue of normal and genetically modified mice permits detailed analysis of this unique cell type. Here, we describe a robust protocol for the isolation and in vitro expansion of LECs from mouse lung by antibody-based magnetic separation.

Keywords: Cell culture; Cell isolation; Lymphatic endothelial cells; Magnetic separation; Mouse tissue.

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Proliferation / drug effects
Cell Proliferation / physiology
Cell Separation / methods*
Cell Survival / drug effects
Cell Survival / physiology
Culture Media / chemistry
Endothelial Cells / cytology*
Endothelial Cells / metabolism
Immunomagnetic Separation / methods*
In Vitro Techniques
Lung / cytology*
Lung / drug effects
Lung / metabolism
Mice

Substances

Culture Media

Grants and funding

R01 HL120888/HL/NHLBI NIH HHS/United States