Identification for surrogate drought tolerance in maize inbred lines utilizing high-throughput phenomics approach

PLoS One. 2021 Jul 27;16(7):e0254318. doi: 10.1371/journal.pone.0254318. eCollection 2021.

Abstract

Screening for drought tolerance requires precise techniques like phonemics, which is an emerging science aimed at non-destructive methods allowing large-scale screening of genotypes. Large-scale screening complements genomic efforts to identify genes relevant for crop improvement. Thirty maize inbred lines from various sources (exotic and indigenous) maintained at Dryland Agriculture Research Station were used in the current study. In the automated plant transport and imaging systems (LemnaTec Scanalyzer system for large plants), top and side view images were taken of the VIS (visible) and NIR (near infrared) range of the light spectrum to capture phenes. All images were obtained with a thermal imager. All sensors were used to collect images one day after shifting the pots from the greenhouse for 11 days. Image processing was done using pre-processing, segmentation and flowered by features' extraction. Different surrogate traits such as pixel area, plant aspect ratio, convex hull ratio and calliper length were estimated. A strong association was found between canopy temperature and above ground biomass under stress conditions. Promising lines in different surrogates will be utilized in breeding programmes to develop mapping populations for traits of interest related to drought resilience, in terms of improved tissue water status and mapping of genes/QTLs for drought traits.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • Biomass
  • Crops, Agricultural
  • Droughts*
  • Genotype
  • Image Processing, Computer-Assisted
  • Phenotype
  • Plant Shoots / growth & development
  • Plant Shoots / physiology
  • Quantitative Trait Loci
  • Water / physiology
  • Zea mays / genetics
  • Zea mays / growth & development
  • Zea mays / physiology*

Substances

  • Water

Grants and funding

We acknowledge the support from INSA, New Delhi, ICAR-NIASM, Baramati, SKUAST-K, Srinagar and IIMR-Ludhiana. This work was supported by the projects VEGA 1/0589/19 “Phenotyping of crop genetic resources for conditions of climatic extremes” and APVV-18-0465 “Use of Advanced Phenomic Approaches to Exploit Variation in Photosynthetic Efficiency to Increase Yield Under Fluctuating and Stress Environment”. This project was supported by Researchers Supporting Project Number (RSP-2021/5) King Saud University, Riyadh, Saudi Arabia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.