Temporal triggers of N2O emissions during cyclical and seasonal variations of a full-scale sequencing batch reactor treating municipal wastewater

Abstract

To investigate the major triggers of nitrous oxide (N₂O) production in a full-scale wastewater treatment plant, N₂O emissions and wastewater characteristics (ammonia, nitrite, nitrate, total nitrogen, dissolved inorganic carbon, dissolved organic carbon, pH, temperature, dissolved oxygen and specific oxygen uptake rate), the results of variations in the cycling of a sequential batch reactor (SBR, where only full nitrification was performed), were monitored seasonally for 16 months. Major triggers of N₂O production were investigated based on a seasonal measured database using a random forest (RF) model and sensitivity analysis, which was applied to identify important input variables. As the result of seasonal monitoring in the full-scale SBR, the N₂O emission factor relative to daily total nitrogen removal ranged from 0.05 to 2.68%, corresponding to a range of N₂O production rate from 0.02 to 0.70 kg-N/day. Results from the RF model and sensitivity analysis revealed that emissions during nitrification were directly or indirectly related to nitrite accumulation, temperature, ammonia loading rate and the specific oxygen uptake rate ratio between ammonia oxidizing bacteria and nitrite oxidizing bacteria (sOUR-ratio). However, changes in the microbial community did not significantly impact N₂O emissions. Based on these results, the sOUR-ratio could represent the major trigger for N₂O emission in a full-scale BNR system: a higher sOUR-ratio value with an average of 3.13 ± 0.23 was linked to a higher N₂O production rate with an average value of 1.27 ± 0.12 kg-N/day (corresponding to 3.96 ± 1.20% of N₂O emission factor relative to daily TN removal), while a lower sOUR-ratio with an average value of 2.39 ± 0.27 was correlated with a lower N₂O production average rate of 0.17 ± 0.11 kg-N/day (corresponding to 0.74 ± 0.69% of N₂O emission factor) (p-value = 0.00001, Mann-Whitney test).

Keywords: Full-scale biological nitrogen removal process; Influential factor; Nitrous oxide; Seasonal measurement; Sequential batch reactor; sOUR-ratio.