Transplantation of Pluripotent Stem Cell-Derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey

Methods Mol Biol. 2021:2320:295-302. doi: 10.1007/978-1-0716-1484-6_25.

Abstract

Recent evidence has provided exciting proof of concepts for the use of pluripotent stem cell-derived cardiomyocytes (PSC-CMs) for cardiac repair; however, large animal studies, which better reflect human disease, are required for clinical application. Here, we describe how to create myocardial infarction in cynomolgus monkey followed by transplantation of PSC-CMs. This method ensures the establishment of a myocardial infarction model and enables reliable PSC-CM transplantation.

Keywords: Cynomolgus monkey; Left anterior descending coronary artery; Myocardial infarction; Pluripotent stem cell-derived cardiomyocyte; Transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anesthesia, Inhalation / methods
  • Anesthesia, Inhalation / veterinary
  • Animals
  • Atropine / therapeutic use
  • Bradycardia / drug therapy
  • Bradycardia / prevention & control
  • Cells, Cultured
  • Disease Models, Animal*
  • Induced Pluripotent Stem Cells / cytology*
  • Intraoperative Complications / drug therapy
  • Intraoperative Complications / prevention & control
  • Ligation
  • Macaca fascicularis*
  • Myocardial Infarction / therapy*
  • Myocytes, Cardiac / transplantation*

Substances

  • Atropine