A Method for Contraction Force Measurement of hiPSC-Derived Engineered Cardiac Tissues

Yuya Fujiwara; Kohei Deguchi; Kenji Miki; Tomoyuki Nishimoto; Yoshinori Yoshida

doi:10.1007/978-1-0716-1484-6_17

A Method for Contraction Force Measurement of hiPSC-Derived Engineered Cardiac Tissues

Methods Mol Biol. 2021:2320:171-180. doi: 10.1007/978-1-0716-1484-6_17.

Authors

Yuya Fujiwara^{1

2}, Kohei Deguchi^{2

3}, Kenji Miki¹, Tomoyuki Nishimoto^{2

3}, Yoshinori Yoshida⁴

Affiliations

¹ Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
² Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.
³ T-CiRA discovery, Takeda Pharmaceutical Company Limited, Fujisawa, Japan.
⁴ Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. yoshinor@cira.kyoto-u.ac.jp.

PMID: 34302658
DOI: 10.1007/978-1-0716-1484-6_17

Abstract

Engineered cardiac tissue (ECT) derived from human induced pluripotent stem cells (iPSCs) can replicate human heart in vitro and be applied to drug discovery and heart disease models. The contraction force of ECT is an important indicator of its function and of the disease phenotype. Here we describe a construction method of ECT using the Flexcell^® Tissue Train^® culture system and a contraction force measurement method based on the Frank-Starling law.

Keywords: Cardiomyocytes; Contraction force; Engineered cardiac tissue; Frank–Starling law; Human induced pluripotent stem cells; Twitch force.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Humans
Induced Pluripotent Stem Cells / cytology*
Myocardial Contraction / physiology*
Myocytes, Cardiac / cytology*
Tissue Engineering / methods*