High Performance of Biohydrogen Production in Packed-Filter Bioreactor via Optimizing Packed-Filter Position

Int J Environ Res Public Health. 2021 Jul 13;18(14):7462. doi: 10.3390/ijerph18147462.

Abstract

In this present investigation, a packed-filter bioreactor was employed to produce hydrogen utilizing an expired soft drink as a substrate. The effects of feeding substrate concentrations ranging from 19.51, 10.19, 5.34, 3.48, to 2.51 g total sugar/L were examined, and the position of the packed filter installed in the bioreactor at dimensionless heights (h/H) of 1/4, 2/4, 3/4, and 4/4 was studied. The results revealed that with a substrate concentration of 20 g total sugar/L and a hydraulic retention time (HRT) of 1 h, a packed filter placed at the half-height position of the bioreactor (h/H 2/4) has the optimal hydrogen production rate, hydrogen yield, and average biomass concentration in the bioreactor, resulting in 55.70 ± 2.42 L/L/d, 0.90 ± 0.06 mol H2/mol hexose, and 17.86 ± 1.09 g VSS/L. When feeding substrate concentrations varied from 20, 10, to 5 g total sugar/L with the packed-filter position at h/H 2/4, Clostridium sp., Clostridium tyrobutyricum, and Bifidobacterium crudilactis were the predominant bacteria community. Finally, it was discovered that the packed-filter bioreactor can produce stable hydrogen in high-strength organic effluent.

Keywords: Clostridium sp.; expired beverage drink; high-strength organic effluent; hydraulic retention time (HRT); hydrogen production rate; hydrogen yield.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bifidobacterium*
  • Bioreactors*
  • Fermentation
  • Hydrogen

Substances

  • Hydrogen

Supplementary concepts

  • Bifidobacterium crudilactis