Characterization and expression pattern of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase gene families in Populus

Yuhan Gao; Xiaoyu Yang; Xiong Yang; Tianyun Zhao; Xinmin An; Zhong Chen

doi:10.1016/j.ijbiomac.2021.07.096

Characterization and expression pattern of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase gene families in Populus

Int J Biol Macromol. 2021 Sep 30:187:9-23. doi: 10.1016/j.ijbiomac.2021.07.096. Epub 2021 Jul 21.

Authors

Yuhan Gao¹, Xiaoyu Yang², Xiong Yang³, Tianyun Zhao⁴, Xinmin An², Zhong Chen⁵

Affiliations

¹ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Forestry, Beijing Forestry University, Beijing 100083, China; Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China; National Energy R&D Center for Non-food Biamass, Beijing Forestry University, Beijing 100083, China.
² National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
³ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Forestry, Beijing Forestry University, Beijing 100083, China; Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China. Electronic address: xiongyang@bjfu.edu.cn.
⁴ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Forestry, Beijing Forestry University, Beijing 100083, China; Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China.
⁵ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Forestry, Beijing Forestry University, Beijing 100083, China; Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China; National Energy R&D Center for Non-food Biamass, Beijing Forestry University, Beijing 100083, China. Electronic address: zhongchen@bjfu.edu.cn.

PMID: 34298047
DOI: 10.1016/j.ijbiomac.2021.07.096

Abstract

Trehalose plays an important role in plant metabolism, growth development, and stress tolerance. Trehalose-6-phosphate synthase gene (TPS) and trehalose-6-phosphate phosphatase gene (TPP) are vital for the synthesis of trehalose. Populus is a prominent perennial woody plant, in which systematic genome-wide analysis of the TPS and TPP family is limited. In this study, 13 PtTPS and 10 PtTPP genes were identified in the Populus genome. Phylogenetic analysis indicated PtTPS and PtTPP genes were both divided into two subfamilies, and gene members of each subfamily have highly conserved intron structures. Analysis of cis-acting elements showed that PtTPS and PtTPP genes were involved in plant hormones and environmental stress responses. Expression profiles also found PtTPSs and PtTPPs expressed differently in response to salt stress, cold, mechanical damage, salicylic acid, and methyl jasmonate treatment. Furthermore, reverse transcription quantitative real-time PCR results found PtTPSs and PtTPPs displayed a specific expression pattern in the seven developmental stages of Populus male and female floral buds. This work will not only lead a foundation on reveal the functions of PtTPS and PtTPP gene families in trehalose regulation of poplar but also provide references to related trehalose research in other perennial plants.

Keywords: Expression pattern; Floral bud; Populus; Trehalose-6-phosphate phosphatase gene; Trehalose-6-phosphate synthase gene.

MeSH terms

Gene Expression Regulation, Enzymologic*
Gene Expression Regulation, Plant*
Genome-Wide Association Study
Glucosyltransferases* / biosynthesis
Glucosyltransferases* / genetics
Multigene Family*
Phosphoric Monoester Hydrolases* / biosynthesis
Phosphoric Monoester Hydrolases* / genetics
Phylogeny
Plant Proteins* / biosynthesis
Plant Proteins* / genetics
Populus* / enzymology
Populus* / genetics

Substances

Plant Proteins
Glucosyltransferases
trehalose-6-phosphate synthase
trehalose-phosphatase
Phosphoric Monoester Hydrolases