Metabolic iron detection through divalent metal transporter 1 and ferroportin mediated cocktail fluorogenic probes

Yu-Qiang Zhao; Jiahong Duan; Le Yu; Ilwha Kim; Yumin Wang; Yi-Cheng Ma; Cheng-Gang Zou; Ying Zhou; Jun Feng Zhang; Jong Seung Kim

doi:10.1039/d1cc03150c

Metabolic iron detection through divalent metal transporter 1 and ferroportin mediated cocktail fluorogenic probes

Chem Commun (Camb). 2021 Aug 10;57(64):7902-7905. doi: 10.1039/d1cc03150c.

Authors

Yu-Qiang Zhao¹, Jiahong Duan², Le Yu³, Ilwha Kim⁴, Yumin Wang⁵, Yi-Cheng Ma², Cheng-Gang Zou², Ying Zhou¹, Jun Feng Zhang⁵, Jong Seung Kim⁴

Affiliations

¹ College of Chemical Science and Technology, Yunnan University, Kunming 650091, P. R. China. yingzhou@ynu.edu.cn.
² State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan 650091, China.
³ College of Chemical Science and Technology, Yunnan University, Kunming 650091, P. R. China. yingzhou@ynu.edu.cn and Department of Chemistry, Korea University, Seoul 02841, Korea. jongskim@korea.ac.kr.
⁴ Department of Chemistry, Korea University, Seoul 02841, Korea. jongskim@korea.ac.kr.
⁵ College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, P. R. China. junfengzhang78@126.com.

PMID: 34286752
DOI: 10.1039/d1cc03150c

Abstract

A cocktail [1 + 2] dual-fluorescent probe system was developed to realize the real-time visualization of dynamic iron state changes between Fe2+ and Fe3+ at the cellular level and in multicellular organisms, providing insights into the effect of DMT1 and ferroportin on iron regulation.

MeSH terms

Cation Transport Proteins / chemistry
Cation Transport Proteins / metabolism*
Fluorescent Dyes / chemistry*
Fluorescent Dyes / metabolism
Hep G2 Cells
Humans
Iron / analysis*
Iron / metabolism
Molecular Structure

Substances

Cation Transport Proteins
Fluorescent Dyes
metal transporting protein 1
Iron