Functional Evaluation of the Signal Peptides of Secreted Proteins

Weixiao Yin; Yufu Wang; Tao Chen; Yang Lin; Chaoxi Luo

doi:10.21769/BioProtoc.2839

Functional Evaluation of the Signal Peptides of Secreted Proteins

Bio Protoc. 2018 May 5;8(9):e2839. doi: 10.21769/BioProtoc.2839.

Authors

Weixiao Yin¹, Yufu Wang¹, Tao Chen¹, Yang Lin¹, Chaoxi Luo¹

Affiliation

¹ College of Plant Science and Technology, Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, and Key Lab of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, China.

Abstract

Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using different selective media and color reaction. In this protocol, we provide the detailed description of manipulation in order to implement easily.

Keywords: Function; Secreted protein; Signal peptide; Yeast; pSUC2 vector.