Adhesion of Enteroaggregative E. coli Strains to HEK293 Cells

Jorge Luis Ayala-Lujan; Fernando Ruiz-Perez

doi:10.21769/BioProtoc.2802

Adhesion of Enteroaggregative E. coli Strains to HEK293 Cells

Bio Protoc. 2018 Apr 20;8(8):e2802. doi: 10.21769/BioProtoc.2802.

Authors

Jorge Luis Ayala-Lujan¹, Fernando Ruiz-Perez²

Affiliations

¹ Unidad Academica de Ciencias Quimicas, Universidad Autonoma de Zacatecas, Zacatecas, Mexico.
² Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia, United States of America.

Abstract

Enteroaggregative Escherichia coli (EAEC) is a recognized cause of acute diarrhea among both children and adults worldwide. EAEC strains are characterized by the presence of aggregative adherence fimbriae (AAF), which play a key role in pathogenesis by mediating attachment to the intestinal mucosa and by triggering host inflammatory responses. The aggregative adherence fimbria II (AAF/II) is the most important adherence factor of EAEC prototype strain 042 (EAEC042) to intestinal cells. Multiple receptors for AAF/II on epithelial cells have been identified including the transmembrane signaling mucin Muc1. This protocol describes a method to measure adherence of EAEC strains to HEK293 cells expressing the Muc1 glycoprotein.

Keywords: Adherence; EAEC; Fimbria; Glycoprotein; Hek293; Muc1; Mucins.