A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila

Kunal Chakraborty; K VijayRaghavan; Rajesh Gunage

doi:10.21769/BioProtoc.2860

A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila

Bio Protoc. 2018 May 20;8(10):e2860. doi: 10.21769/BioProtoc.2860.

Authors

Kunal Chakraborty^{1

2}, K VijayRaghavan¹, Rajesh Gunage^{1

3}

Affiliations

¹ Department of Developmental Biology and Genetics, National Centre for Biological Sciences, Bangalore, India.
² School of Chemical & Biotechnology, Shanmugha Arts, Science, Technology & Research Academy, Thanjavur, India.
³ Stem Cell Program and Division of Haematology/Oncology, Children's Hospital, Howard Hughes Medical Institute, Boston, United States.

Abstract

Inducing an injury specifically to Drosophila flight muscles is a difficult task, owing to the small size of the muscles and the presence of the cuticle. The protocol described below provides an easy and reproducible method to induce injury in the Drosophila flight muscles.

Keywords: Drosophila flight muscles; Injury; Insect satellite cells; Regeneration.