Genetic characteristics of OXA-48-producing Enterobacterales from China

Lifeng Wang; Ling Guo; Kun Ye; Jiyong Yang

doi:10.1016/j.jgar.2021.07.006

Genetic characteristics of OXA-48-producing Enterobacterales from China

J Glob Antimicrob Resist. 2021 Sep:26:285-291. doi: 10.1016/j.jgar.2021.07.006. Epub 2021 Jul 18.

Authors

Lifeng Wang¹, Ling Guo¹, Kun Ye¹, Jiyong Yang²

Affiliations

¹ Department of Laboratory Medicine, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
² Department of Laboratory Medicine, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China. Electronic address: yangjy301@163.com.

PMID: 34284128
DOI: 10.1016/j.jgar.2021.07.006

Abstract

Objectives: In China, OXA-48-producing Enterobacterales have been identified sporadically, causing small-scale regional outbreaks. This study investigated the molecular epidemiology, transmission and evolution of OXA-48-producing Enterobacterales and pOXA48 from mainland China.

Methods: We conducted a multicentre resistance monitoring project from 2013-2018. Genome sequencing of OXA-48-produicng isolates was performed. SNPs were analysed. Eleven isolates were selected for subsequent SMRT sequencing. Genome sequences were annotated, and alignment of the complete sequences of bla_OXA-48-carrying plasmids from a subset of isolates that underwent long-read sequencing was performed.

Results: In total, 41 OXA-48-producing Enterobacterales were included in this study (34 Klebsiella pneumoniae, 3 Escherichia coli, 3 Enterobacter cloacae complex and 1 Klebsiella oxytoca). OXA-48-produicng K. pneumoniae (OXAKp) ST383, ST147 and ST11 caused outbreaks of different scales in our hospital. OXA-48-producing E. coli ST156 and ST648, E. cloacae complex ST414 and ST418, and K. oxytoca ST34 were also identified. bla_OXA-48 was embedded in a Tn1999.2 structure located in IncL plasmids with different sizes (63.58-109.14 kb). Importantly, K. pneumoniae ST11 co-producing KPC-2 and OXA-48 was identified in our hospital and it is possible that KPC-2-producing K. pneumoniae ST11 obtained the bla_OXA-48-carrying plasmid during its spread. A novel 109-kb bla_OXA-48-carrying IncL plasmid was identified from OXAKp ST11. Possibly, two plasmids in OXAKp ST383 were integrated to form this larger plasmid.

Conclusion: OXA-48-produicng Enterobacterales were sporadic in China. Importantly, K. pneumoniae ST11 co-producing KPC-2 and OXA-48 has emerged causing an outbreak in China. This high-risk multidrug-resistant clone exhibited high compatibility and strong integration ability with foreign resistance plasmids.

Keywords: Carbapenemase; Enterobacterales; Genomics; Klebsiella pneumoniae; OXA-48.

MeSH terms

Escherichia coli / genetics
Humans
Klebsiella Infections*
Klebsiella pneumoniae / genetics
Plasmids / genetics
beta-Lactamases / genetics

Substances

beta-Lactamases