Litoribacter populi sp. nov., isolated from the soil of a Populus euphratica forest

Abstract

A Gram-stain-negative, rod-shaped, and orange-pigmented bacterium, designated XAAS-A1^T, was isolated from the soil of a Populus euphratica forest. The main respiratory quinone of XAAS-A1^T is MK-7, and the principal cellular fatty acids are iso-C_15:0, C_17:1 ω6c, Iso-C_17:0 3OH, and summed feature 3 (C_16:1ω6c and/or C_16:1ω7c). The major polar lipids are phosphatidylethanolamine, phosphatidylcholine, and two unidentified lipids. The 16S rRNA gene sequence analysis indicated that XAAS-A1^T belongs to the genus Litoribacter within the family Cyclobacteriaceae, having the highest sequence similarities to Litoribacter ruber KCTC 22899^T (97.1%) and L. alkaliphilus KCTC 32217^T (96.9%). XAAS-A1^T has a DNA G + C content of 42.1 mol%. The orthoANI and the digital DNA-DNA hybridization values were markedly lower than the recommended threshold values of 96% and 70%. Phenotypic and genotypic data suggest that XAAS-A1^T represents a novel species of the genus Litoribacter, for which we propose the name Litoribacter populi sp. nov. and XAAS-A1^T (= CCTCC AB 2017159^T = KCTC 62045^T) as the type strain.

Keywords: Cyclobacteriaceae; Litoribacter; Populus euphratica; Soil.