Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets

STAR Protoc. 2021 Jul 3;2(3):100650. doi: 10.1016/j.xpro.2021.100650. eCollection 2021 Sep 17.

Abstract

Platelet preparations are commonly used in the clinic in combination with mesenchymal stem cells (MSCs) to improve their wound healing capacity and optimize their therapeutic efficacy following their delivery into diseased tissues. To investigate the mechanisms by which platelets enhance the repair properties of MSCs, we detail a protocol using a humanized mouse model for excisional wounds to study by reverse transcription real-time PCR whether human platelets alter the therapeutic efficacy of grafted human MSCs. For complete details on the use and execution of this protocol, please refer to Levoux et al. (2021).

Keywords: Cell Biology; Health Sciences; Metabolism; Model Organisms; Molecular Biology; Stem Cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Platelets
  • Gene Expression Profiling / methods*
  • Heterografts
  • Humans
  • Male
  • Mesenchymal Stem Cell Transplantation / methods*
  • Mice
  • Mice, Inbred C57BL
  • Platelet Transfusion / methods*
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Wounds, Penetrating / genetics
  • Wounds, Penetrating / therapy*