A novel fluorescent probe for the detection of peroxynitrite and its application in acute liver injury model

Chen Jin; Pengfei Wu; Yushun Yang; Zhenxiang He; Hailiang Zhu; Zhen Li

doi:10.1016/j.redox.2021.102068

A novel fluorescent probe for the detection of peroxynitrite and its application in acute liver injury model

Redox Biol. 2021 Oct:46:102068. doi: 10.1016/j.redox.2021.102068. Epub 2021 Jul 10.

Authors

Chen Jin¹, Pengfei Wu², Yushun Yang¹, Zhenxiang He³, Hailiang Zhu⁴, Zhen Li⁵

Affiliations

¹ State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China.
² Pancreas Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, 210029, China; Pancreas Institute of Nanjing Medical University, Nanjing, 210029, China.
³ State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China. Electronic address: zxhe@nju.edu.cn.
⁴ State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China. Electronic address: zhuhl@nju.edu.cn.
⁵ State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China. Electronic address: lizhenzfx@nju.edu.cn.

Abstract

Realizing the early diagnosis of acute liver injury has become a hotspot in recent years. Since the current indexes are not specific, developing novel probes with new recognition group remains a necessity. In this work, we developed a novel fluorescent probe, NNP, for the detection of ONOO^- in acute liver injury (ALI). The in vitro evaluation of NNP indicated the advantages including high sensitivity (LOD = 0.13 μM), rapid response (<25 s), naked-eye (red to yellow), and stability under various pH (4-10) and time (>48 h) conditions. Accordingly, NNP achieved the dose-dependent detection of ONOO^- in human hepatic stellate LX-2 cells. Further, in the ALI model mice, NNP could monitor the ONOO^- level in the occurrence of ALI in situ (in the liver region) with a steady performance. Subsequent immunohistochemical and imaging studies confirmed that NNP could achieve the detection of endogenous ONOO^- in ALI liver tissues. This work introduced a practical implement for the ONOO^- detection in ALI as well as a referable example for the establishment of molecular indexes in pre-clinical diagnosis.

Keywords: Acute liver injury; Fluorescent probe; In vivo imaging; Peroxynitrite detection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fluorescent Dyes*
Liver
Mice
Peroxynitrous Acid*

Substances

Fluorescent Dyes
Peroxynitrous Acid