Lanthanum(III) triggers AtrbohD- and jasmonic acid-dependent systemic endocytosis in plants

Mengzhu Cheng; Lihong Wang; Qing Zhou; Daiyin Chao; Shingo Nagawa; Ding He; Jiazhi Zhang; Hui Li; Li Tan; Zhenhong Gu; Xiaohua Huang; Zhenbiao Yang

doi:10.1038/s41467-021-24379-z

Lanthanum(III) triggers AtrbohD- and jasmonic acid-dependent systemic endocytosis in plants

Nat Commun. 2021 Jul 15;12(1):4327. doi: 10.1038/s41467-021-24379-z.

Authors

Mengzhu Cheng^#¹, Lihong Wang^#^{1

2}, Qing Zhou², Daiyin Chao³, Shingo Nagawa^{4

5}, Ding He², Jiazhi Zhang², Hui Li^{5

6}, Li Tan⁵, Zhenhong Gu⁵, Xiaohua Huang⁷, Zhenbiao Yang^{8

9}

Affiliations

¹ National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
³ National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
⁴ Fujian Agriculture and Forestry University-University of California, Riverside, Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China.
⁵ Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
⁶ School of Life Sciences, East China Normal University, Shanghai, China.
⁷ National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, China. huangxiaohuanjnu@yahoo.com.
⁸ Fujian Agriculture and Forestry University-University of California, Riverside, Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China. yang@ucr.edu.
⁹ Department of Botany and Plant Sciences, Center for Plant Cell Biology, Institute of Integrative Genome Biology, University of California, Riverside, CA, USA. yang@ucr.edu.

^# Contributed equally.

Abstract

Trivalent rare earth elements (REEs) are widely used in agriculture. Aerially applied REEs enter leaf epidermal cells by endocytosis and act systemically to improve the growth of the whole plant. The mechanistic basis of their systemic activity is unclear. Here, we show that treatment of Arabidopsis leaves with trivalent lanthanum [La(III)], a representative of REEs, triggers systemic endocytosis from leaves to roots. La(III)-induced systemic endocytosis requires AtrbohD-mediated reactive oxygen species production and jasmonic acid. Systemic endocytosis impacts the accumulation of mineral elements and the development of roots consistent with the growth promoting effects induced by aerially applied REEs. These findings provide insights into the mechanistic basis of REE activity in plants.

Publication types

Research Support, Non-U.S. Gov't
Video-Audio Media

MeSH terms

Arabidopsis / cytology
Arabidopsis / drug effects*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Cyclopentanes / metabolism
Endocytosis / drug effects*
Endocytosis / physiology
Gene Expression Regulation, Plant
Green Fluorescent Proteins / genetics
Lanthanum / pharmacology*
Minerals / metabolism
NADPH Oxidases / genetics
NADPH Oxidases / metabolism*
Oxylipins / metabolism
Plant Cells / drug effects
Plant Leaves / cytology
Plant Leaves / drug effects
Plant Roots / cytology
Plant Roots / drug effects
Plant Roots / metabolism
Plants, Genetically Modified
Signal Transduction

Substances

Arabidopsis Proteins
Cyclopentanes
Minerals
Oxylipins
Green Fluorescent Proteins
Lanthanum
jasmonic acid
respiratory burst oxidase homolog D, Arabidopsis
NADPH Oxidases