Bioremediation of ossein effluents using the filamentous marine cyanobacterium Cylindrospermum stagnale

Abstract

Wastewater containg proteinaceous ossein effluents are problematic to be treated. We studied the possibility to treat ossein effluents with the marine cyanobacterium strain Cylindrospermum stagnale. After optimizing the culture conditions of the bacterium, three different types of ossein effluents were tested: dicalcium phosphate (DCP), high total dissolved solids (HTDS) and low total dissolved (LTDS). The effluents were diluted with sea water at the following ratios 1:1, 2:1 and 3:2. The optimum operating conditions were at 3000 lux light intensity and 37 °C temperature. The highest degradation of ossein effluens by C. stagnale was attained for a dilution ratio of 1:1. However, less diluted ossein effluents reduced the growth of C. stagnale drastically. The degradation was shown by measuring the chlorophyll a content and the dry weight of bacterial cells during a seven-day incubation period degradation. Fourier Transform Infrared Spectroscopy (FT-IR) analysis verified the degradation showing the presence of the degradation products of ossein (i.e. calcium carbonate and calcite) in the culture medium. Lipid composition in fatty acids appeared to be suitable for biofuel production. The results showed that the marine cyanobacterium C. stagnale can be used to treat ossein effluents, and at the same time, to produce biofuel in a sustainable way.

Keywords: Cylindrospermum stagnale; FT-IR analysis; Fatty acid analysis; Marine cyanobacteria; Ossein effluent.