Manganese methionine hydroxy analog chelated affects growth performance, trace element deposition and expression of related transporters of broilers

Tiantian Meng; Lumin Gao; Chunyan Xie; Yangkui Xiang; Yiqiang Huang; Yawei Zhang; Xin Wu

doi:10.1016/j.aninu.2020.09.005

Manganese methionine hydroxy analog chelated affects growth performance, trace element deposition and expression of related transporters of broilers

Anim Nutr. 2021 Jun;7(2):481-487. doi: 10.1016/j.aninu.2020.09.005. Epub 2021 Mar 11.

Authors

Tiantian Meng^{1

2}, Lumin Gao¹, Chunyan Xie², Yangkui Xiang³, Yiqiang Huang³, Yawei Zhang³, Xin Wu^{1

2

4}

Affiliations

¹ CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, 410125, China.
² Hunan Co-Innovation Center of Safety Animal Production, College of Animal Science and Technology; College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China.
³ Hunan Provincial Research Center of Mineral Element Nutrition Engineering Technology, Xing-Jia Bio-engineering Co., Ltd., 410300, Changsha, China.
⁴ Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Abstract

The present study aimed to evaluate the effects of manganese methionine hydroxyl analog chelated (Mn-MHAC) as a manganese (Mn) source on growth performance and trace element deposition in broilers. A total of 432 Arbor Acres commercial female broilers were fed a basal corn-soybean diet containing Mn at 25.64 mg/kg diet for 10 d. They were then randomly assigned to 6 groups, including a control group (the basal diet), a Mn sulfate group (the basal diet supplemented with Mn at 100 mg/kg diet), and 4 Mn-MHAC groups (the basal diet supplemented with Mn-MHAC at 25, 50, 75 and 100 mg Mn/kg diet, respectively). The results showed that compared with the control group, groups supplemented with Mn-MHAC had a positive effect on BW (quadratic, P = 0.017) and ADG (quadratic, P = 0.017). Moreover, the Mn-MHAC (50 mg Mn/kg diet) group had significantly greater BW and ADG (P < 0.05) compared with the other Mn-MHAC groups. Trace element deposition results also showed that tibial Mn increased (linear or quadratic, P = 0.002 and 0.009, respectively) in groups fed diets with increased levels of Mn-MHAC. In contrast, Fe deposition decreased both in the heart (linear, P = 0.020) and tibia (P < 0.05). In addition, the Mn-MHAC supplement noticeably lowered serum Mn-SOD activity (linear or quadratic, P = 0.048 and 0.019, respectively). The relative mRNA levels of divalent metal transporter 1 (DMT1) (P = 0.024), ferroportin 1 (FPN1) (P = 0.049), and Cu transporter-1(CTR1) (P < 0.001) in the duodenum, as well as CTR1 in the jejunum and ileum (P = 0.040 and 0.011, respectively) were higher in the Mn-supplemented group than in the control group. Furthermore, the relative mRNA level of DMT1 in the jejunum and ileum of broilers in the Mn-MHAC group (50 mg Mn/kg diet) did not differ from those in the control group, but was lower than those in the Mn sulfate group (P < 0.05). In conclusion, Mn-MHAC dietary supplementation improved the growth performance and trace element deposition in broilers. From this study, we recommend the optimum Mn-MHAC level to meet the Mn requirement of broilers is 50 to 75 mg Mn/kg diet.

Keywords: Broiler; Deposition; Manganese methionine hydroxy analog chelated; Trace element; Transporter.