Genomic and proteomic comparisons of bacteriocins in probiotic species Lactobacillus and Bifidobacterium and inhibitory ability of Escherichia coli MG 1655

Nasrin Darvishi; Najaf Allahyari Fard; Maryam Sadrnia

doi:10.1016/j.btre.2021.e00654

Genomic and proteomic comparisons of bacteriocins in probiotic species Lactobacillus and Bifidobacterium and inhibitory ability of Escherichia coli MG 1655

Biotechnol Rep (Amst). 2021 Jun 22:31:e00654. doi: 10.1016/j.btre.2021.e00654. eCollection 2021 Sep.

Authors

Nasrin Darvishi¹, Najaf Allahyari Fard², Maryam Sadrnia³

Affiliations

¹ Payame Noor University (PNU), National Institute of Genetic Engineering and Biotechnology, Iran.
² National Institute of Genetic Engineering and Biotechnology (NIGEB), Iran.
³ Department of Biology, Payame Noor University (PNU), Tehran, Iran.

Abstract

Bacteriocins are a large family of bacterial peptides or proteins, ribosomally synthesized with antimicrobial activity against other bacteria. We investigated and compared the genomes and proteomes of 12 Bifidobacterium and 46 Lactobacillus species for bacteriocins using NCBI-Genome, UniProt-Proteome, Bactibase, and BAGL4 databases. Selected Lactobacillus species were examined for bile salt resistance, acid and pH resistance, pepsin and trypsin enzyme resistance, and antibiotic resistance. Also, the antimicrobial activity of selected Lactobacillus species was evaluated against E. coli MG 1655. Results showed that Lactobacillus species have more diversity and abundance of bacteriocin compared to Bifidobacterium species. Notably, L. sakei, L. plamtarum, L. reuteri, L. fermentum, and L. casei had the highest pathogen inhibition; respectively. Therefore, a combination of these Lactobacillus species can be suggested as a biochemical and safe solution to control gastrointestinal pathogens and suitable alternatives to antibiotics and chemicals in food technology.

Keywords: Bacteriocin; Genome; Pathogen inhibition; Probiotics; Proteome.