A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes

Jun Zi Zhu; Jun Guo; Zhao Hu; Xu Tong Zhang; Xiao Gang Li; Jie Zhong

doi:10.3389/fmicb.2021.653809

A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes

Front Microbiol. 2021 Jun 22:12:653809. doi: 10.3389/fmicb.2021.653809. eCollection 2021.

Authors

Jun Zi Zhu^{1

2}, Jun Guo², Zhao Hu², Xu Tong Zhang², Xiao Gang Li^{1

2}, Jie Zhong²

Affiliations

¹ Hunan Engineering Research Center of Agricultural Pest Early Warning and Control, Hunan Agricultural University, Changsha, China.
² Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, China.

Abstract

Here, we report a novel double-stranded RNA virus designated Colletotrichum liriopes partitivirus 1 (ClPV1) from the plant pathogenic fungus C. liriopes. ClPV1 genome has two double stranded RNAs (dsRNAs), named as dsRNA 1 and dsRNA 2, which in the lengths of 1,807 and 1,706 bp, respectively. The dsRNA 1 and dsRNA 2 encoded proteins showing significant amino acid (aa) sequence identity to the RNA-dependent RNA polymerase (RdRp) and coat protein (CP) of partitiviruses, respectively. Phylogenetic analysis using the aa sequences of RdRp and CP indicated that ClPV1 was grouped to members of the putative Epsilonpartitivirus genus in the Partitiviridae family. Spherical viral particles in approximately 35 nm in diameter and packaging the ClPV1 genome were isolated. Virus elimination and virus transfection with purified viral particles, and biological comparison revealed that ClPV1 could reduce the virulence and conidia production of C. liriopes. To the best of our knowledge, this is the first report of mycovirus in C. liriopes fungus.

Keywords: Colletotrichum liriopes; Colletotrichum liriopes partitivirus 1; dsRNA; hypovirulence; partitivirus.