Forests play an important role in the global carbon (C) balance, but their biomass has decreased globally mainly because of deforestation and a reduction in forest cover. However, little is known about the C stock of tree biomass related to environmental factors in the remnant forest patches. Thus, the present study aimed at assessing the status of C stocks of tree biomass using an allometric equation in Kibate Forest (Ethiopia). Sixty-six plots (30×30 m) were laid out at 100 m interval distance along the altitudinal gradients in five transects. The results revealed that the highest C stocks (67.4%) per species were contributed by Juniperus procera, Ilex mitis var. mitis, Nuxia congesta, and Olea europaea subsp. cuspidata. The mean total tree biomass was 91.9 ± 10.01 Mg ha-1. The mean total C stock was 45.9 ± 5.17 Mg ha-1, out of which 38.3 ± 4.31 and 7.7 ± 0.91 Mg ha-1 were stored in above- and belowground C pools, respectively. Anthropogenic factors were negatively associated with the C-stock distribution in the study area. Thus, the status of the C stock of tree biomass related to anthropogenic factors indicates that sustainable forest management practice is needed in the study area to conserve biodiversity and mitigate climate change.