A protocol for human bone marrow adipocyte isolation and purification

Camille Attané; David Estève; Mohamed Moutahir; Nicolas Reina; Catherine Muller

doi:10.1016/j.xpro.2021.100629

A protocol for human bone marrow adipocyte isolation and purification

STAR Protoc. 2021 Jun 24;2(3):100629. doi: 10.1016/j.xpro.2021.100629. eCollection 2021 Sep 17.

Authors

Camille Attané^{1

2}, David Estève^{1

2}, Mohamed Moutahir^{1

2}, Nicolas Reina^{3

4}, Catherine Muller^{1

2}

Affiliations

¹ Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS UMR 5089, 205 Route de Narbonne, 31077 Toulouse, France.
² Equipe labellisée Ligue contre le cancer, Toulouse, France.
³ Département de Chirurgie Orthopédique et Traumatologique, Hôpital Pierre-Paul Riquet, CHU de Toulouse, 31059 Toulouse, France.
⁴ Laboratoire AMIS, UMR 5288 CNRS, Université Toulouse III-Paul Sabatier, 31060 Toulouse, France.

Abstract

Primary human bone marrow adipocytes (BM-Ads) display a specific metabolism that is not recapitulated by in vitro differentiated bone marrow mesenchymal stromal cells. These findings highlight the need for using primary BM-Ads in studies of the metabolic impact of BM-Ads on surrounding cells. Here, we present a protocol for isolating human BM-Ads from bone marrow aspirates and verifying adipocyte suspension purity. These isolated and purified BM-Ads can be used for functional assays or frozen for molecular analyses. For complete details on the use and execution of this protocol, please refer to Attane et al. (2020).

Keywords: Cancer; Cell Biology; Cell isolation; Metabolism; Microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / cytology*
Bone Marrow Cells / cytology*
Femur / cytology
Fluorescent Antibody Technique
Humans
Reproducibility of Results